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Published online before print February 11, 2008
RNA, DOI: 10.1261/rna.779908
 Copyright © 2008 RNA Society
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pH-dependent structural changes of helix 69 from Escherichia coli 23S ribosomal RNA

Sanjaya C. Abeysirigunawardena and Christine S. Chow

Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA

Helix 69 in 23S rRNA is a region in the ribosome that participates in a considerable number of RNA–RNA and RNA–protein interactions. Conformational flexibility is essential for such a region to interact and accommodate protein factors at different stages of protein biosynthesis. In this study, pH-dependent structural and stability changes were observed for helix 69 through a variety of spectroscopic techniques, such as circular dichroism spectroscopy, UV melting, and nuclear magnetic resonance spectroscopy. In Escherichia coli 23S rRNA, helix 69 contains pseudouridine residues at positions 1911, 1915, and 1917. The presence of these pseudouridines was found to be essential for the pH-induced conformational changes. Some of the pH-dependent changes appear to be localized to the loop region of helix 69, emphasizing the importance of the highly conserved nature of residues in this region.

Keywords: helix 69; 23S rRNA; pseudouridine; modified nucleotides

Received August 13, 2007 ; accepted January 11, 2008.

Reprint requests to: Christine S. Chow, Department of Chemistry, Wayne State University, Detroit, MI 49202, USA; e-mail: csc{at}chem.wayne.edu; fax: (313) 577-8822.

Article published online ahead of print. Article and publication date are at http://www.rnajournal.org/cgi/doi/10.1261/rna.779908.


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