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Sulfolobus solfataricus translation initiation factor 1 stimulates translation initiation complex formation

¹ Max F. Perutz Laboratories, Department of Microbiology and Immunobiology, University Departments at the Vienna Biocenter, 1030 Vienna, Austria
² Department of Cellular Biotechnology and Hematology, University of Rome, 00161 Rome, Italy
³ Department of Medicinal Biochemistry, Biology and Physics, University of Bari, 70124 Bari, Italy

Reprint requests to: Udo Bläsi, Max F. Perutz Laboratories, Department of Microbiology and Immunobiology, University Departments at the Vienna Biocenter, Dr. Bohrgasse 9/4, 1030 Vienna, Austria; e-mail: Udo.Blaesi{at}univie.ac.at; fax: +43-1-4277-9546.

The eukaryotic translation initiation factor 1 binds to the ribosome during translation initiation. It is instrumental for initiator-tRNA and mRNA binding, and has a function in selection of the authentic start codon. Here, we show that the archaeal homolog aIF1 has analogous functions. The aIF1 protein of the archaeon Sulfolobus solfataricus is bound to the small ribosomal subunit during translation initiation and accelerates binding of initiator-tRNA and mRNA to the ribosome. Accordingly, aIF1 stimulated translation of an mRNA in a S. solfataricus in vitro translation system. Moreover, this study suggested that the C terminus of the factor is of relevance for its function.

Keywords: aIF1; archaea; Sulfolobus solfataricus; translation initiation

⁴ These authors contributed equally to this work.

Article published online ahead of print. Article and publication date are at http://www.rnajournal.org/cgi/doi/10.1261/rna.2289306.

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