| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742, USA
2 Program in Molecular Biosciences at University of Medicine and Dentistry of New Jersey (UMDNJ)/Rutgers Universities, Piscataway New Jersey 08854, USA
Reprint requests to: Jonathan D. Dinman, Department of Cell Biology and Molecular Genetics, Microbiology Building, Room 2135, University of Maryland, College Park, MD 20742, USA; e-mail: dinman{at}umd.edu; fax: 301-314-9489.
Increased efficiencies of programmed -1 ribosomal frameshifting in yeast cells expressing mutant forms of ribosomal protein L3 are unable to maintain the dsRNA "Killer" virus. Here we demonstrate that changes in frameshifting and virus maintenance in these mutants correlates with decreased peptidyltransferase activities. The mutants did not affect Ty1-directed programmed +1 ribosomal frameshifting or nonsense-mediated mRNA decay. Independent experiments demonstrate similar programmed -1 ribosomal frameshifting specific defects in cells lacking ribosomal protein L41, which has previously been shown to result in peptidyltransferase defects in yeast. These findings are consistent with the hypothesis that decreased peptidyltransferase activity should result in longer ribosome pause times after the accommodation step of the elongation cycle, allowing more time for ribosomal slippage at programmed -1 ribosomal frameshift signals.
Keywords: Frameshifting; ribosome; virus
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Meskauskas, J. R. Russ, and J. D. Dinman Structure/function analysis of yeast ribosomal protein L2 Nucleic Acids Res., April 1, 2008; 36(6): 1826 - 1835. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Vassilaki, K. I. Kalliampakou, and P. Mavromara Differences in the expression of the hepatitis C virus core+1 open reading frame between a nuclear and a cytoplasmic expression system J. Gen. Virol., January 1, 2008; 89(1): 222 - 231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. V. Rosado, D. Kressler, and J. d. l. Cruz Functional analysis of Saccharomyces cerevisiae ribosomal protein Rpl3p in ribosome synthesis Nucleic Acids Res., June 13, 2007; (2007) gkm388v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mansouri, E. Nourollahzadeh, and K. A. Hudak Pokeweed antiviral protein depurinates the sarcin/ricin loop of the rRNA prior to binding of aminoacyl-tRNA to the ribosomal A-site RNA, September 1, 2006; 12(9): 1683 - 1692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Muldoon-Jacobs and J. D. Dinman Specific effects of ribosome-tethered molecular chaperones on programmed -1 ribosomal frameshifting. Eukaryot. Cell, April 1, 2006; 5(4): 762 - 770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Meskauskas, A. N. Petrov, and J. D. Dinman Identification of Functionally Important Amino Acids of Ribosomal Protein L3 by Saturation Mutagenesis Mol. Cell. Biol., December 15, 2005; 25(24): 10863 - 10874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Makelainen and K. Makinen Factors affecting translation at the programmed -1 ribosomal frameshifting site of Cocksfoot mottle virus RNA in vivo Nucleic Acids Res., April 20, 2005; 33(7): 2239 - 2247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Jacobs and J. D. Dinman Systematic analysis of bicistronic reporter assay data Nucleic Acids Res., November 23, 2004; 32(20): e160 - e160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. LEGER, S. SIDANI, and L. BRAKIER-GINGRAS A reassessment of the response of the bacterial ribosome to the frameshift stimulatory signal of the human immunodeficiency virus type 1 RNA, August 1, 2004; 10(8): 1225 - 1235. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
