HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: rna.518107v1 13/6/930    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Maroney, P. A. Articles by Nilsen, T. W. Search for Related Content PubMed PubMed Citation Articles by Maroney, P. A. Articles by Nilsen, T. W. Social Bookmarking                   What's this?

A rapid, quantitative assay for direct detection of microRNAs and other small RNAs using splinted ligation

¹ Center for RNA Molecular Biology and Department of Biochemistry, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106-4973, USA
² USB Corporation, Cleveland, Ohio 44128, USA

The discovery and characterization of microRNAs (miRNAs) and other families of short RNAs has led to a rapid expansion of research directed at elucidating their expression patterns and regulatory functions. Here, we describe a convenient, sensitive, and straightforward method to detect and quantitate specific miRNA levels in unfractionated total RNA samples. The method, based on splinted ligation, does not require specialized equipment or any amplification step, and is significantly faster and more sensitive than Northern blotting. We demonstrate that the method can be used to detect various classes of small regulatory RNAs from different organisms.

Keywords: miRNA; microRNA; small RNA; splinted ligation; Northern blot

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Lu, D.-H. Jeong, K. Kulkarni, M. Pillay, K. Nobuta, R. German, S. R. Thatcher, C. Maher, L. Zhang, D. Ware, et al. Genome-wide analysis for discovery of rice microRNAs reveals natural antisense microRNAs (nat-miRNAs) PNAS, March 25, 2008; 105(12): 4951 - 4956. [Abstract] [Full Text] [PDF]

				X. Tang, J. Gal, X. Zhuang, W. Wang, H. Zhu, and G. Tang A simple array platform for microRNA analysis and its application in mouse tissues RNA, October 1, 2007; 13(10): 1803 - 1822. [Abstract] [Full Text] [PDF]