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Transcriptional termination sequences in the mouse serum albumin gene

¹ Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
² Cell and Developmental Biology Program, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA

Reprint requests to: Nick J. Proudfoot, Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK; e-mail: Nicholas.proudfoot{at}path.ox.ac.uk; fax: +01865-275556.

Poly(A) signals are required for efficient 3' end formation and transcriptional termination of most protein-encoding genes transcribed by RNA polymerase II. However, transcription can extend far beyond the poly(A) site before termination occurs. This implies the existence of further downstream termination signals. In mammals, a variety of sequence elements, in addition to the poly(A) site, have been implicated in the termination process. For example, termination of the human ß- and -globin genes is mediated by a sequence downstream of the poly(A) site that promotes an RNA cotranscriptional cleavage (CoTC). Here we report the identification of multiple termination sequences in the mouse serum albumin (MSA) 3' flanking region. Many transcripts from this region are cleaved cotranscriptionally, implying that such cleavage of pre-mRNA may be a more general feature of transcriptional termination.

Keywords: albumin; transcriptional termination; poly(A) signal; cotranscriptional cleavage

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